VTU´s Pichia pastoris protein expression platform

By applying VTU´s Pichia pastoris protein expression technology researchers at VTU have repeatedly demonstrated their ability to rapidly produce a wide range of different proteins (serum proteins, cytokines, Fabs and other antibody derived fragments, scaffold proteins, HSA-fusion proteins, Fc-fusion proteins and enzymes) by full exploitation of VTU´s protein expression technologies and optimized procedures for high-throughput expression strain development. VTU´s expression technology platform is based on the following technological advances: 1. Proprietary technology - VTU´s exclusive AOX1 promoter libraries provide an unprecedented genetic diversity with superior regulatory properties for fine tuning of gene expression by selecting the perfect match of promoter/target combinations. 2. FTO tools - VTU´s in-house modular plasmid system and platform strains enable the optimization of protein production and provide more flexibility to fulfill the different requirements of various expression tasks. 3. Optimized procedures - VTU´s team applies highly elaborated and time-saving procedures, based on a pool approach for transformation and a high-throughput cultivation - & screening regime allowing for maximization of output. Key components of VTU´s Pichia pastoris protein expression platform